Screening for vitamin D status – our method

There are various methods to assess stores of vitamin D in your body and we use best practices to ensure our accuracy of analysis is superior. A 25-hydroxyvitamin D test is the best way to monitor vitamin D levels as it measures the 25(OH)D, also called calcidiol, in your blood. Vitamin D must go through several transformation processes before your body can use it and calcidiol is the chemical that your body first converts vitamin D into when it gets to the liver.

The test reflects all three sources of vitamin D; from food, supplements and skin synthesis, and determines if your vitamin D levels are optimal, too high or too low. Samples are analyzed by Vitas, our contract laboratory in Norway, using liquid chromatography followed by mass spectrum.

The range of vitamin D is measured as nanograms per milliliter (ng/mL) or nanomoles per liter (nmol/L). nmol/L stands for nanomoles per liter and is the most common way of showing the vitamin D status. However, in the US, and a handful of other countries, the same levels are measured using nanograms per milliliter (or ng/mL). The two differing units of measurement are somewhat like metric vs imperial. A liter measures fluid volume.

nmol/L: A mole is an amount of a substance that contains a large number (6 followed by 23 zeros) of molecules or atoms – as such, a nanomole is one billionth of a mole.

ng/mL: The nanogram is equal to one billionth of a gram (10-9g). 1 nanogram per milliliter [ng/mL] = 0.001 milligram per liter [mg/L].

For ease of comparison:
1 nmol/L = 0.4 ng/mL
1 ng/mL = 2.5 nmol/L

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Screening for vitamin D status – our method